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Influenza

A rapid test for the qualitative detection of influenza type A and type B antigens in nasal swab specimens.

  • 98.9% Accuracy
  • CE self-test | MHRA Certified
  • Specimen Type: Nasal Swab
About the Test

The Influenza Test, (Influenza A+B rapid test) is a rapid chromatographic immunoassay for the qualitative detection of influenza A and B antigens in nasopharyngeal swab, throat swab or nasal aspirate specimens. It is intended to aid in the rapid differential diagnosis of influenza A and B viral infections.

In this test, antibodies specific to the Influenza A and Influenza B nucleoproteins are separately coated on the test line regions of the test dipstick. During testing, the extracted specimen reacts with the antibody to Influenza A and/or Influenza B that are coated onto particles. The specimen migrates up the membrane to react with the antibody to Influenza A and/or Influenza B on the membrane and generates one or two coloured lines in the test regions. The presence of this coloured line in either or both of the test regions indicates a positive result. To serve as a procedural control, a coloured line will always appear in the control region if the test has performed properly.

Influenza, commonly known as ‘flu’, is a highly contagious, acute viral infection of the respiratory tract. It occurs worldwide and spreads very quickly in populations. In the northern hemisphere, annual influenza epidemics affect approximately 5-15% of the population. Influenza is a communicable disease easily transmitted through the coughing and sneezing of aerosolised droplets containing live virus. Influenza outbreaks occur each year during the autumn months. Type A viruses are typically more prevalent than type B viruses and are associated with most serious influenza epidemics, while type B infections are usually milder.

Instructions

Specimen collection and preparation

Nasopharyngeal swab sample:

1. Insert a sterile swab into the nostril of the patient, reaching the surface of the posterior nasopharynx.

2. Swab over the surface of the posterior nasopharynx 5-10 times.

Throat swab sample

Insert a sterilised swab into the pharynx and collect mucoepidermoid, mainly wiping the flare region of the post-pharyngeal wall and palatine tonsil several times, and be careful not to make saliva attach to the swab.

Nasal aspirate

Connect an aspiration catheter to an aspiration trap that is attached to an aspiration device, insert the catheter to the nasal cavity from a nostril, start the aspiration device and then collect nasal aspirate sample. Dip a sterilised swab into the collected nasal aspirate sample and make the specimen cling to the swab.

Procedure

Allow the test, specimen, extraction buffer to equilibrate to room temperature (15-30°C) prior to testing.

1. Remove the test dipstick from the sealed foil pouch and use it as soon as possible. Best results will be obtained if the assay is performed immediately after opening the foil pouch.

2. Place the extraction tube in the workstation. Hold the extraction reagent bottle upside down vertically. Squeeze the bottle and let the solution drop into the extraction tube freely without touching the edge of the tube. Add 10 drops of extraction reagent (approx 400μl) to the extraction tube.

3. Place the swab specimen in the extraction tube. Rotate the swab for approximately 10 seconds while pressing the head against the inside of the tube to release the antigen in the swab.

4. Remove the swab while squeezing the swab head against the inside of the extraction tube as you remove it, to expel as much liquid as possible from the swab. Discard the swab securely.

5. With arrows pointing down, place the dipstick into the tube of solution, and then start the timer. If the procedure is followed correctly, the liquid should be below the maximum line on the test dipstick.

6. Wait for the coloured line(s) to appear. Read the result at 15 minutes. Do not interpret the result after 20 minutes.

Reading the Results

Read the result at 15 minutes. Do not interpret the result after 20 minutes.

POSITIVE Influenza A: Two distinct coloured lines appear. One coloured line should be in the control region (C) and another coloured line should be in the Influenza A region (A). A positive result in the Influenza A region indicates that Influenza A antigen was detected in the sample.

POSITIVE Influenza B: Two distinct coloured lines appear. One coloured line should be in the control region (C) and another coloured line should be in the Influenza B region (B). A positive result in the Influenza B region indicates that Influenza B antigen was detected in the sample.

POSITIVE Influenza A and Influenza B: Three distinct coloured lines appear. One coloured line should be in the control region (C) and two coloured line should be in the Influenza A region (A) and Influenza B region (B). A positive result in the Influenza A region and Influenza B region indicates that Influenza A antigen and Influenza B antigen were detected in the sample.

NOTE: The intensity of the colour in the test line regions (A or B) will vary based on the amount of Influenza A or B antigen present in the sample. So any shade of colour in the test regions (A or B) should be considered positive.

NEGATIVE: One coloured line appears in the control region (C). No apparent coloured line appears in the test line regions (A or B).

INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test with a new test dipstick. If the problem persists, discontinue using the test kit immediately and contact your local distributor.

Book In Clinic

in clinic Test £15.00 per test

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Order a Postal Test Kit £70.00

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In Clinic Influenza Test

A rapid test for the qualitative detection of influenza type A and type B antigens in nasal swab specimens.

Buy your Postal Influenza Test

Postal Influenza Test

A rapid test for the qualitative detection of influenza type A and type B antigens in nasal swab specimens.